Mechanism of Centaureidin-Induced Apoptosis in A549 Cells

Objective To investigate the effect of centaureidin on the proliferation and apoptosis of A549 cells and its mechanism of action.Methods The proliferation of A549 cells was measured by MTT assay,the apoptosis by Hochest staining,the apoptosis rate and cell cycle by flow cytometry,and the expression of NF-κB P65 by Western blot.Results Centaureidin treatment suppressed proliferation,induced apoptosis and up-regulated NF-κB P65 expression in A549 cells in a dose-dependent manner.The median inhibitory concentration(IC50)was 0.67μmol·L-1.G1 phase arrest was found in A549 cells treated with centaureidin at a concentration of 0.15or0.30μmol·L-1.Conclusion Centaureidin can induce apoptosis in A549 cells via activating NF-κB signal pathway.