基质凝胶
层粘连蛋白
血管生成
细胞外基质
体内
佩莱肯
细胞生物学
基质(化学分析)
化学
体外
生物
生物化学
免疫学
癌症研究
蛋白多糖
遗传学
色谱法
作者
Pinelopi Kastana,Fatema Tuz Zahra,Δέσποινα Ντενέκου,Stamatiki Katraki-Pavlou,Dimitris Beis,Michail S. Lionakis,Constantinos M. Mikelis,Evangelia Papadimitriou
出处
期刊:Methods in molecular biology
日期:2019-01-01
卷期号:: 219-232
被引量:40
标识
DOI:10.1007/978-1-4939-9133-4_18
摘要
Matrigel is extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma in C57BL/6 mice, a tumor rich in extracellular matrix (ECM) proteins. It consists mainly of laminin (approximately 60%), collagen IV (approximately 30%), and nidogen-1/entactin (approximately 8%), while it also contains heparan sulfate proteoglycans, such as perlecan, other ECM proteins, as well as growth factors bound to the ECM. Matrigel mimics the physiological cell matrix and is the most commonly used matrix substrate to study in vitro and in vivo angiogenesis. Here, we describe the in vivo Matrigel plug assay and how it can be used for both qualitative and quantitative assessment of angiogenesis.
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