乳过氧化物酶
化学
阿布茨
牛乳
过氧化氢
食品科学
检出限
硫氰酸盐
色谱法
荧光
过氧化物酶
生物化学
抗氧化剂
酶
物理
量子力学
DPPH
作者
Zhengzheng Zou,Julien Bauland,Amitha K. Hewavitharana,Saad S. Al-Shehri,John A. Duley,David Cowley,Pieter Koorts,P. Nicholas Shaw,Nidhi Bansal
出处
期刊:Food Chemistry
[Elsevier]
日期:2021-03-01
卷期号:339: 128090-128090
被引量:16
标识
DOI:10.1016/j.foodchem.2020.128090
摘要
Lactoperoxidase (LPO) is one of the major antibacterial ingredients in milk and an extensively employed indicator for milk heat treatment. The traditional method for LPO activity measurement using ABTS (2,2′-azinobis(3-ethylbenzothiazoline-6-sulphonate) cannot achieve high sensitivity and is affected by indigenous milk thiocyanate. A more sensitive microplate fluorescent assay was developed by monitoring generation of red-fluorescent resorufin from LPO catalysed oxidation of Amplex® Red (1-(3,7-dihydroxyphenoxazin-10-yl)ethanone) in this study. The assay is particularly suitable for milk LPO activity measurement as it eliminates the influences of indigenous milk hydrogen peroxide and thiocyanate. The method limit of detection was 7.1x10−6 U/mL of LPO in milk and good intra-run and inter-run precision was obtained. The LPO activities ranked as bovine > goat > camel > human in the four types of milk analysed. The high sensitivity and low cost of this assay makes it suitable for LPO activity analyses in both laboratory and commercial scales.
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