污染
核酸
核酸检测
次氯酸钠
化学
色谱法
生物
有机化学
生物化学
生态学
作者
Yunlong Li,Jian Zhang,Yanqiu Wei,Jia Xiao-juan,Xiaoyan Li,Liming Tan,Wenjun Liu,Limin Yang
出处
期刊:Chinese Journal of Biotechnology
日期:2021-02-25
卷期号:37 (2): 673-679
被引量:1
标识
DOI:10.13345/j.cjb.200390
摘要
Nucleic acid detection technique has good sensitivity and specificity and is widely used in in vitro diagnosis, animal and plant commodity quarantine, forensic identification, and other fields. However, it is susceptible to carryover contamination during the operation and leads to false-positive results, which seriously affects the detection accuracy. Therefore, finding an effective solution to prevent and eliminate nucleic acid carryover contamination has become particularly urgent. This study compared several different methods for removing nucleic acid contamination and confirmed that sodium hypochlorite solution and PCRguard reagent could effectively eliminate nucleic acid carryover in the liquid and on surfaces of different materials. Besides, the combination of sodium hypochlorite solution and PCRguard can solve the nucleic acid aerosol contamination. This study proposes solutions for the routine prevention of carryover contamination and removal of aerosol that has occurred in molecular diagnostic laboratories.
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