变构调节
结合位点
变构调节剂
毒蕈碱乙酰胆碱受体
化学
跨膜结构域
可药性
生物
受体
生物物理学
生物化学
基因
作者
Wessel A. C. Burger,Patrick R. Gentry,Alice E. Berizzi,Ziva Vuckovic,Emma T. Westhuizen,Geoff Thompson,Mahmuda Yeasmin,Craig W. Lindsley,Patrick M. Sexton,Christopher J. Langmead,Andrew B. Tobin,Arthur Christopoulos,Céline Valant,David M. Thal
标识
DOI:10.1021/acschemneuro.1c00383
摘要
The M5 muscarinic acetylcholine receptor (mAChR) has emerged as an exciting therapeutic target for the treatment of addiction and behavioral disorders. This has been in part due to promising preclinical studies with the M5 mAChR selective negative allosteric modulator (NAM), ML375. The binding site of ML375 remains unknown, however, making it difficult to develop improved M5 mAChR selective modulators. To determine the possible location of the ML375 binding site, we used radioligand binding and functional assays to show that ML375 does not interact with the well-characterized "common" mAChR allosteric site located in the receptor's extracellular vestibule, nor a previously proposed second allosteric site recognized by the modulator, amiodarone. Molecular docking was used to predict potential allosteric sites within the transmembrane (TM) domain of the M5 mAChR. These predicted sites were assessed using M5–M2 mAChR receptor chimeras and further targeted with site-directed mutagenesis, which enabled the identification of a putative binding site for ML375 at the interface of TMs 2–4. Collectively, these results identify a third allosteric site at the M5 mAChR and highlight the ability of allosteric modulators to selectively target highly conserved proteins.
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