脾脏
接种
肺结核
传输(电信)
病毒学
接种疫苗
生物
结核病疫苗
牛分枝杆菌
持久性(不连续性)
结核分枝杆菌
微生物学
医学
免疫学
病理
电气工程
工程类
岩土工程
作者
Ximena Ferrara Muñiz,Elizabeth A. García,Federico C. Blanco,Fabiana Bigi,Martín J. Zumárraga,Angel A. Cataldi,María Emilia Eirin
出处
期刊:Tuberculosis
[Elsevier]
日期:2022-03-01
卷期号:: 102203-102203
标识
DOI:10.1016/j.tube.2022.102203
摘要
Cattle vaccination is an attractive approach in compliance with control and eradication programs against Bovine Tuberculosis (bTB). Today, there is no anti bTB vaccine licensed. Two vaccine candidates, MbΔmce2 and MbΔmce2-phoP previously designed were evaluated in BALB/c mice, including the parental M. bovis NCTC10772 and a M. bovis hypervirulent Mb04-303 strains as controls. Sentinel mice (non-inoculated) cohoused with subcutaneous inoculated mice. Persistence, visible tuberculosis lesions (VTL) in lungs and spleens and bacillary load were investigated subcutaneously delivered at 60 and 90 days after inoculation (dpi) as well as their potential transmission to naïve mice. While a 100% survival was observed at 90 dpi without VTL in all groups, transmission was not evidenced in the sentinels mice. Vaccine candidates and control strains were isolated from the spleen of all inoculated mice, while Mb04-303 was isolated from the lungs of one inoculated mouse. Vaccine candidate's attenuation considering survival, lung bacillary load and VTL was confirmed, administrated by the subcutaneous route. Future experiments are necessary to demonstrate whether the persistence of both mutants in the spleen, with low CFU, remains over time to increase the potential increasing risk of dissemination to organs and subsequent transmission to other animals by airborne or other routes.
科研通智能强力驱动
Strongly Powered by AbleSci AI