亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Ganluyin ameliorates DSS-induced ulcerative colitis by inhibiting the enteric-origin LPS/TLR4/NF-κB pathway

封堵器 炎症性肠病 溃疡性结肠炎 杯状细胞 结肠炎 TLR4型 脂多糖 紧密连接 医学 NF-κB 粘液 肠粘膜 污渍 炎症 免疫学 内科学 病理 生物 上皮 疾病 生物化学 基因 生态学
作者
Taoxiu Xiong,Xiang Zheng,Ke Zhang,Han-Song Wu,Yingjie Dong,Fu-Chen Zhou,Bingbing Cheng,Linzi Li,Wanfeng Xu,Jie Shi,Jian Huang,Zetian Jiang,Bo Li,Beibei Zhang,Gui‐Yuan Lv,Suhong Chen
出处
期刊:Journal of Ethnopharmacology [Elsevier]
卷期号:289: 115001-115001 被引量:42
标识
DOI:10.1016/j.jep.2022.115001
摘要

Ulcerative colitis (UC) is a chronic inflammatory bowel disease (IBD), that is associated with a significantly increased risk of colon cancer. As a classic traditional Chinese medicine, Ganluyin (GLY) has a long history as an anti-inflammatory medication, but its impacts on UC has not been established.This study aims to evaluate the protective effect and mechanism of GLY on a pathway involving enteric-origin lipopolysaccharide (LPS), toll-like receptor (TLR)4, and NF-κB in mice with dextran sulfate sodium (DSS)-induced UC.After three weeks of intragastric administration of GLY, a UC model was induced in mice by administration of 4% DSS in drinking water for one week. The disease activity index (DAI) was measured, and histological staining was used to detect histopathological changes of colon. LPS content of the serum was measured by ELISA, and the expression of tight junction proteins and proteins related to TLR4/NF-κB pathway in colon were analyzed by immunohistochemistry or Western Blotting. The intestinal flora was analyzed by 16S rRNA sequencing.GLY improved the histological pathological changes of DSS-induced UC, as assessed by DAI, colonic mucosal damage, inflammatory cell infiltration, and goblet cell and mucus reduction. GLY also protected the intestinal mucosal barrier by increasing the expression of the tight junction proteins, occludin, claudin-1, and ZO-1 and by reducing the serum LPS content and decreasing the expression of TLR4, MyD88, NF-κB, IL-6, IL-1β, and TNF-α proteins in colon. Analyses of the intestinal flora showed that GLY restored the homeostasis of the intestinal flora through increases in the abundance of Firmicutes and decreases in the abundance of Proteobacteria and Bacteroidetes, which is associated with the production of LPS.GLY might exert an anti-UC effect by improving the colonic mucosal barrier and inhibiting the enteric-origin LPS/TLR4/NF-κB inflammatory pathway, and restoring the homeostasis of the intestinal flora in UC mice. These discoveries lay a strong foundation for GLY as a UC treatment.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
我是老大应助kke采纳,获得10
1秒前
lena发布了新的文献求助10
2秒前
4秒前
6秒前
sdfzaq1完成签到,获得积分10
7秒前
niko发布了新的文献求助10
8秒前
丁丁发布了新的文献求助10
10秒前
sdfzaq1发布了新的文献求助10
10秒前
完美世界应助lena采纳,获得10
16秒前
哈哈完成签到 ,获得积分10
19秒前
完美世界应助丁丁采纳,获得10
20秒前
研友_Y58465完成签到,获得积分10
21秒前
23秒前
可爱的小桃完成签到,获得积分10
25秒前
升升升呀应助英俊的海安采纳,获得40
36秒前
温水煮青蛙完成签到 ,获得积分10
52秒前
大东东发布了新的文献求助10
58秒前
大东东完成签到,获得积分10
1分钟前
能干的元龙完成签到 ,获得积分10
1分钟前
1分钟前
pcr163给somebodyzou的求助进行了留言
1分钟前
1分钟前
在水一方应助科研通管家采纳,获得10
1分钟前
小蘑菇应助科研通管家采纳,获得10
1分钟前
执着的难破完成签到 ,获得积分10
1分钟前
852应助坦率的金鱼采纳,获得10
1分钟前
羊羊发布了新的文献求助10
1分钟前
开放素完成签到 ,获得积分10
1分钟前
Tsingyuan发布了新的文献求助30
1分钟前
1分钟前
DSUNNY完成签到 ,获得积分10
1分钟前
淳于穆发布了新的文献求助30
1分钟前
羊羊完成签到,获得积分10
1分钟前
2分钟前
SciGPT应助昵昵昵昵呀采纳,获得10
2分钟前
小二郎应助淳于穆采纳,获得10
2分钟前
2分钟前
2分钟前
2分钟前
尹不愁应助糖糖采纳,获得20
2分钟前
高分求助中
Un calendrier babylonien des travaux, des signes et des mois: Séries iqqur îpuš 1036
Sustainable Land Management: Strategies to Cope with the Marginalisation of Agriculture 1000
Corrosion and Oxygen Control 600
Heterocyclic Stilbene and Bibenzyl Derivatives in Liverworts: Distribution, Structures, Total Synthesis and Biological Activity 500
重庆市新能源汽车产业大数据招商指南(两链两图两池两库两平台两清单两报告) 400
Division and square root. Digit-recurrence algorithms and implementations 400
行動データの計算論モデリング 強化学習モデルを例として 400
热门求助领域 (近24小时)
化学 材料科学 医学 生物 有机化学 工程类 生物化学 纳米技术 物理 内科学 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 电极 光电子学 量子力学
热门帖子
关注 科研通微信公众号,转发送积分 2545225
求助须知:如何正确求助?哪些是违规求助? 2175592
关于积分的说明 5600078
捐赠科研通 1896314
什么是DOI,文献DOI怎么找? 946171
版权声明 565327
科研通“疑难数据库(出版商)”最低求助积分说明 503541