化学
膜
荧光
生物物理学
荧光显微镜
双光子激发显微术
活体细胞成像
小泡
荧光寿命成像显微镜
显微镜
细胞
生物化学
病理
医学
物理
量子力学
生物
作者
Yingcui Bu,Mengtao Rong,Junjun Wang,Xiaojiao Zhu,Jie Zhang,Lianke Wang,Zhipeng Yu,Yupeng Tian,Hongping Zhou,Yi Xie
标识
DOI:10.1021/acs.analchem.2c00874
摘要
Various suborganelles are delimited by lipid bilayers, in which high spatial and temporal morphological changes are essential to many physiological and pathological processes of cells. However, almost all the amphiphilic fluorescent molecules reported until now are not available for in situ precise tracking of membrane dynamics in cell apoptosis. Here, the MO (coumarin pyridine derivatives) was devised by engineering lipophilic coumarin and cationic pyridine salt, which not only lastingly anchored onto the plasma membrane in dark due to appropriate amphipathicity and electrostatic interactions but also in situ reflected the membrane damage and heterogeneity with secretion of extracellular vesicles (EVs) under reactive oxygen species regulation and was investigated by two-photon fluorescence lifetime imaging microscopy. This work opens up a new avenue for the development of plasma membrane staining and EV-based medicines for the early diagnosis and treatment of disease.
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