生物反应器
反硝化细菌
颗粒
反硝化
废水
化学
制浆造纸工业
生物降解
污水处理
流出物
硝酸盐
化学需氧量
微生物
工业废水处理
发酵
环境化学
海藻酸钙
黑曲霉
色谱法
硝化作用
水力停留时间
生物修复
材料科学
环境工程
氮气
有机化学
环境科学
复合材料
作者
Zhi-Jie Zheng,Amjad Ali,Junfeng Su,Tinglin Huang,Yue Wang,Shuai Zhang
标识
DOI:10.1016/j.biortech.2021.125113
摘要
In this study, fungal pellets immobilized denitrifying Pseudomonas stutzeri sp. GF3 was cultivated to establish a bioreactor. The denitrification effect of fixed bacteria with fungal pellets was tested by response surface methodology (RSM). Analysis of the bioreactor showed that the denitrification efficiency reached 100% under the optimal conditions and the denitrification efficiency of the actual wastewater treatment in the stable phase reached 95.91%. Moreover, the organic matter and functional groups in the bioreactor under different C/N conditions were analyzed by fluorescence excitation-emission matrix (EEM) spectra and Fourier transform infrared spectroscopy (FTIR), which revealed that metabolic activities of denitrifying bacteria were enhanced with the increase of C/N. The morphology and structure of bacteria immobilized by fungal pellets explored by scanning electron microscope (SEM) showed the filamentous porous fungal pellets loaded with bacteria. Community structure analysis by high-throughput sequencing demonstrated that strain GF3 might was the dominant strain in bioreactor.
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