诱导多能干细胞
胚芽层
人诱导多能干细胞
干细胞
细胞生物学
生物
细胞分化
定向微分
药物发现
胚胎干细胞
生物信息学
遗传学
基因
作者
Giovanni Giuseppe Giobbe,Federica Michielin,Camilla Luni,Stefano Giulitti,Sebastian Martewicz,Sirio Dupont,Annarosa Floreani,Nicola Elvassore
出处
期刊:Nature Methods
[Springer Nature]
日期:2015-07-01
卷期号:12 (7): 637-640
被引量:109
摘要
Microengineering human "organs-on-chips" remains an open challenge. Here, we describe a robust microfluidics-based approach for the differentiation of human pluripotent stem cells directly on a chip. Extrinsic signal modulation, achieved through optimal frequency of medium delivery, can be used as a parameter for improved germ layer specification and cell differentiation. Human cardiomyocytes and hepatocytes derived on chips showed functional phenotypes and responses to temporally defined drug treatments.
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