化学
DPPH
食品科学
胡萝卜素
阿布茨
发酵
抗氧化剂
固态发酵
β-胡萝卜素
环境污染
橙色(颜色)
高效液相色谱法
制浆造纸工业
色谱法
类胡萝卜素
有机化学
环境科学
工程类
环境保护
作者
Prabhjot Kaur,Gargi Ghoshal,Ashay Jain
标识
DOI:10.1016/j.procbio.2018.10.007
摘要
Fruits and vegetable processing industries produce huge waste in the form of peels, seeds, liquid, and molasses which are a good source of carbohydrates, proteins, fibres, vitamins, and minerals. This waste can be utilized for the production of biocolors using fermentation. Utilization of this waste not only valorizes waste disposal problems but also eliminate environmental pollution. The aim of the present work was to extract and optimize environmental factors for production of β-carotene from fruits and vegetable waste (orange, carrot, and papaya peels) using microbial strain Blakeslea trispora (+) MTCC 884 in solid state fermentation. It was observed that the production of β-carotene was significantly influenced by varying all factors and gave a maximum yield of 0.127 mg/mL. Characterization of the extracted color was done with techniques like HPLC, LCMS, FTIR and Mass Spectroscopy. Mass spectroscopy of extracted color represented the m/z value 537.608 and LCMS analysis gave the eluted peaks at (Rt 13.37), which confirmed the presence of β-carotene. Furthermore, β-carotene percentage estimated by HPLC and LCMS was over 76% suggesting that these fruits and vegetable wastes may be used for the production of β-carotene with high purity and gave good antioxidant properties as determined by DPPH and ABTS.
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