重组酶聚合酶扩增
副溶血性弧菌
沙门氏菌
金黄色葡萄球菌
微生物学
生物
多重聚合酶链反应
肠炎沙门氏菌
多路复用
量油尺
实时聚合酶链反应
病菌
聚合酶链反应
细菌
基因
遗传学
尿
生物化学
作者
Bach Ma,Jiali Li,Kai Chen,Xiaoping Yu,Chuanxin Sun,Mingzhou Zhang
出处
期刊:Foods
[MDPI AG]
日期:2020-03-03
卷期号:9 (3): 278-278
被引量:41
摘要
Foodborne pathogens can cause foodborne illness. In reality, one food sample may carry more than one pathogen. A rapid, sensitive, and multiple target method for bacteria detection is crucial in food safety. For the simultaneous detection of Staphylococcus aureus, Vibrio parahaemolyticus, and Salmonella Enteritidis, multi-objective recombinase polymerase amplification (RPA) combined with a lateral flow dipstick (LFD) was developed in this study. The whole process, including amplification and reading, can be completed in 15 min at 37 °C. The detection limits were 2.6 × 101 CFU/mL for Staphylococcus aureus, 7.6 × 101 CFU/mL for Vibrio parahaemolyticus, and 1.29 × 101 CFU/mL for Salmonella Enteritidis. Moreover, colored signal intensities on test lines were measured by a test strip reader to achieve quantitative detection for Staphylococcus aureus (R2 = 0.9903), Vibrio parahaemolyticus (R2 = 0.9928), and Salmonella Enteritidis (R2 = 0.9945). In addition, the method demonstrated good recoveries (92.00%-107.95%) in the testing of spiked food samples. Therefore, the multiplex LFD-RPA assay is a feasible method for the rapid, sensitive, and quantitative detection of bacterial pathogens in seafood.
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