化学
谷胱甘肽
赫拉
选择性
检出限
多胺
荧光
胎牛血清
生物化学
生物物理学
细胞
色谱法
酶
催化作用
物理
生物
量子力学
作者
Xiaobo Wang,Xiaoyan Ma,Jinghan Wen,Zhirong Geng,Zhilin Wang
出处
期刊:Talanta
[Elsevier]
日期:2020-01-01
卷期号:207: 120311-120311
被引量:22
标识
DOI:10.1016/j.talanta.2019.120311
摘要
Detection of glutathione in human serum is of great importance for clinical diagnosis of various diseases, such as AIDS, diabetes mellitus, Alzheimer disease and cancer. In this work, a new water-soluble bismacrocyclic polyamine-derived compound, namely L, which contains two molecules of 4-nitro-1,2,3-benzoxa-diazole as the fluorophores, was designed and prepared. The experiments of selectivity of L toward metal ions showed it could rapidly and sensitively detect Hg2+ with a detection limit of 27 nM. Furthermore, the cell imaging and co-staining experiments in HeLa cells demonstrated that the L-Hg2+ probe had selectivity for the Golgi apparatus to a certain degree. Moreover, it had excellent selectivity for biothiols, especially for glutathione. Finally, the probe was successfully applied to sensitively detect glutathione (GSH) in human serum and fetal bovine serum.
科研通智能强力驱动
Strongly Powered by AbleSci AI