[Studies on the mechanism of antibody formation. 8. Influence of adrenalin on the in vitro formation of the antibody-promoting substance Inosin].

The present investigation was made to clarify the action of adrenalin on the formation of inosine, which was identified as antibody-promoting factor. The results obtained were as follows.Adrenalin displayed its action at two locations in the course of metabolic degradation of ATP and adenosine. At one location, it accelerated the degradation from ATP or adenosine to inosine and hypoxanthine, and at the other it inhibited the conversion from inosine and hypoxanthine to xanthine. Especially, when adrenalin and adenosine were used together, inosine and hypoxanthine were found to have increased about 1.7 times after incubation for 40-60 minutes, but xanthine did not behave in the same manner. The optimal rate of adrenalin was 100μg per ml of basic medium.