The semen analysis is a very important to evaluate the clinical inspection items of male reproductive ability.However,semen analysis result is not reliable,there are many factors that directly affect the result of the semen analysis when the semen analysis.Through investigation and analysis the major factors affect semen analysis are semen specimen collection way and time,specimen preservation and management,and the specimen test technology and attitude.Only the correct way of collecting semen,suitable semen specimen containers and storage,good inspection staff attitude,can obtain accurate analysis results of semen specimens.