Polyploid Induction of Artemisia rupestris L.by Colchicine

Polyploids were induced from Artemisia rupestris L.by two methods,soaking shoot segments in colchicine solution and adding colchicine in the culture medium,respectively.The results showed that the soaking-treatment increased the efficiency of polyploids induction than the other methods.As to method of soaking-treatment,the optimal condition of treatment was 0.2%colchicine for 1 d and the induction rate of polyploid reached 27.3%.