OBJECTIVE: To analyze volatile oil from Teuerium labiosum,T. bidentatant and T. pilosum. METHODS:Volatile oils were extracted from T. labiosum,T. bidentatant and T. pilosum using solid phase microextraction (SPME). GC-MS was adopted to determine volatile oil. RESULTS: 33 kinds of chemical components were identified in volatile oil of T. bidentatant and T. labiosum. The top 5 chemical components in list of content were Germacrene-D(26.66% and 27.88%), 1-octen-3-ol (13.19% and 13.79%),α-pinene(12.53% and 13.07%),β-selinene(5.93% and 6.08%) and Germacrene-B(5.89%and 6.20%).29 chemical components were identified in volatile oil of T. lusitanicum with Caryophyllene oxide (21.52%),α-bisabolol(20.35%),α-pinene oxide(18.25%),1-octen-3-ol(7.26%),and α-bisabolol oxide B (5.92%) as main constituents. CONCLUSION: The study provide theory basis for exploration of Teuerium.