Observation on the immune effect of subunit vaccine of Toxoplasma gondii with different adjuvants

Objective To observe the immune response and protective immunity in mice induced by P30/35 subunit vaccine of Toxoplasmd gondii with different adjuvants. Methods Mice were immunized with P30/35 subunit vaccine of Toxoplasma gondii which were combined with bacteria-like particles adjuvant,LTA adjuvant,propolis extra adjuvant or CFA,then the immune response and the survival time of mouse attacked by Toxoplasma gondii were observed. Results The highest immune response was induced by P30/35 subunit vaccine combined with bacteria-like particles adjuvant.This vaccine and adjuvant can offer good protective immunity.The IL-2 titre induced by bacteria-like particles adjuvant was lower than that induced by CFA,but highter significantly than other groups(P0.01).The IFN-gamma level and CD 4 +/CD 8 + artio induced by bacteria-like particles adjuvant had no significant difference with that by CFA(P0.05).Furthermore,the IgG titre by bacteria-like particles adjuvant was higher than that induced by CFA(P0.01).The immune response and protective immunity induced by LTA adjuvant and propolis extra adjuvant were lower than that induced by bacteria-like particles adjuvant and CFA. Conclusion The adility of stimulating immunity response of bacteria-like particles adjuvant is as the same as CFA,and this adjuvant can increase the immunogenicity of P30/35 proteins,so this adjuvant can be used on the research of subunit vaccine of Toxoplasma gondii.