Object To study the chemical constituents from the seeds of Sophora japonica L Methods The constituents were isolated and purified by silica gel column chromatography, and the structures were elucidated by spectral data and chemical envidance Results Six compounds were isolated from its alcohol extract and identified as: genistein 7 O β D glucopyranoside 4′ O β D glucopyranoside (Ⅰ), genistein 7 O β D glucopyranoside 4′ O L rhamnopyranosyl (1→2) β D glucopyranoside] (Ⅱ), tectorigenin (Ⅲ), tectoridin (Ⅳ), 1, 6 di (Ⅱ), tectorigenin (Ⅲ), tectoridin (Ⅳ), 1, 6 di O galloyl β D glucose (Ⅴ), ethyl gallate (Ⅵ) Conclusion Among them, compounds Ⅲ Ⅵ were isolated from this plant for the first time