Leachate leakage enhances the microbial diversity and richness but decreases Proteobacteria and weakens stable microbial ecosystem in landfill groundwater

Sanitary landfill is the most prevalent and economic method for municipal solid waste disposal, and the resultant groundwater pollution has become an environmental problem due to leachate leakage. The pollution characteristics in groundwater near landfill sites have been extensively investigated, although the succession characteristics and driving mechanisms of microbial communities in leachate-contaminated groundwater and the sensitive microbial indicators for leachate leakage identification remain poorly studied. Herein, results showed that leachate leakage enhanced the microbial diversity and richness and transferred endemic bacteria from landfills into groundwater, producing an average decrease of 17.73% in the relative abundance of Proteobacteria. The key environmental factor driving the evolution of microbial communities in groundwater due to leachate pollution was organic matter, which can explain 16.13% of the changes in microbial community composition. The |βNTI| values of the bacterial communities in all six landfills were <2, and the assembly process of microbial communities was primarily dominated using stochastic processes. Leachate pollution changed the assembly mechanism, transforming the community assembly process from an undominated process to a dispersal limitation process. Leachate pollution reduced the efficiency and stability of microbial communities in groundwater, increasing the vulnerability of the stable microbial ecosystems in groundwater. Notably, microbial indicators are more sensitive to leachate leakage and could accurately identify landfills where leachate leakage occurred and other extraneous pollutants. The phylum Proteobacteria and mcrA could act as appropriate indicators for the identification of leachate leakage. These results provide a novel insight into the monitoring, identification of groundwater pollution and the scientific guidance for appropriate remediation strategies for leachate-contaminated groundwater.