外周血单个核细胞
生物标志物
免疫系统
基因表达
癌症研究
下调和上调
生物
CXCL10型
医学
免疫学
基因
趋化因子
遗传学
体外
作者
Seo Young Kwak,Ji-Hye Park,Hee-Young Won,Hyosun Jang,Seung Bum Lee,Won Il Jang,Sunhoo Park,Minjung Kim,Sehwan Shim
标识
DOI:10.1080/09553002.2023.2295300
摘要
In case of a nuclear accident, individuals with high-dose radiation exposure (>1-2 Gy) should be rapidly identified. While ferredoxin reductase (FDXR) was recently suggested as a radiation-responsive gene, the use of a single gene biomarker limits radiation dose assessment. To overcome this limitation, we sought to identify reliable radiation-responsive gene biomarkers.Peripheral blood mononuclear cells (PBMCs) were isolated from mice after total body irradiation, and gene expression was analyzed using a microarray approach to identify radiation-responsive genes.In light of the essential role of the immune response following radiation exposure, we selected several immune-related candidate genes upregulated by radiation exposure in both mouse and human PBMCs. In particular, the expression of ACOD1 and CXCL10 increased in a radiation dose-dependent manner, while remaining unchanged following lipopolysaccharide (LPS) stimulation in human PBMCs. The expression of both genes was further evaluated in the blood of cancer patients before and after radiotherapy. CXCL10 expression exhibited a distinct increase after radiotherapy and was positively correlated with FDXR expression.CXCL10 expression in irradiated PBMCs represents a potential biomarker for radiation exposure.
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