Mapping a new secalin locus on the rye 1RS arm

A gene designated a Sec-N encoding a secalin was mapped in the introgressive winter common wheat line Hostianum 273/97 (H273) with the wheat-rye (1B)1R substitution from the octoploid triticale AD825. F2 seeds from crossing the line H273 with the line Hostianum 242/97-2 carrying the wheat-rye 1BL/1RS translocation were analyzed. The studied component on the SDS-electrophoregram of total proteins was revealed to be a monomeric secalin, which is encoded by the gene at the new locus Sec-N located distally with respect to the Sec-1 locus at a distance of 21.4 ± 2.5% (22.9 ± 3.1 cM). The arrangement of the secalin loci on the 1RS arm indicates that the Sec-N locus is to be homoeologous to the Gli-1 loci of common wheat.