Modified electrochemical aptasensor for ultrasensitive detection of tetracycline: In silico and in vitro studies

An ultrasensitive electrochemical aptasensor based on a glassy carbon electrode, modified by carbon nanofibers and carboxylated multi-walled carbon nanotubes was fabricated to detect tetracycline in food samples. The affinity of antibiotics, including kanamycin, tetracycline, ampicillin, and sulfadimethoxine toward desired sequences of aptamers and the stability of antibiotic-aptamer complexes were studied using molecular docking and molecular dynamic simulations. Moreover, the highest affinity and most stable complex were observed for tetracycline in complex with kanamycin-specific aptamer (KAP). Finally, KAP was used to develop an aptasensor. The central composite design (CCD) was used to optimize effective parameters. The biosensor achieved a wide dynamic linear range (1.0 × 10-17-1.0 × 10-5 M) and a low limit of detection (2.28 × 10-18 M) under optimized conditions using differential pulse voltammetry. Using the developed aptasensor, tetracycline residues in milk samples were determined.