化学
纳米团簇
免疫分析
检出限
聚乙烯吡咯烷酮
配体(生物化学)
分散性
纳米颗粒
纳米技术
化学工程
色谱法
高分子化学
有机化学
生物化学
材料科学
受体
抗体
工程类
免疫学
生物
作者
Shuyun Chen,Zhichao Yu,Yunsen Wang,Juan Tang,Yongyi Zeng,Xiaolong Liu,Dianping Tang
出处
期刊:Analytical Chemistry
[American Chemical Society]
日期:2023-09-14
卷期号:95 (38): 14494-14501
被引量:33
标识
DOI:10.1021/acs.analchem.3c03249
摘要
Noble-metal nanozymes have demonstrated great potential in various fields. However, aggregation of single-particle nanoparticles severely affects their exposed catalytically active sites to the extent of exhibiting weak enzyme-like activity. Here, we present an organic block surfactant (polyvinylpyrrolidone, PVP) to construct monodisperse water-stable Pt nanoclusters (Pt NCs) for an enhanced immunoassay of cardiac troponin I (cTnI). The PVP-modified Pt NC nanozyme exhibited up to 16.3 U mg-1 peroxidase-mimicking activity, which was mainly attributed to the ligand modification on the surface and the electron-absorbing effect of the ligand on the Pt NCs. The PVP-modified Pt NCs have a lower OH-transition potential, as determined by density functional theory. Under optimized experimental conditions, the enhanced nanozyme immunoassay strategy exhibited an ultrawide dynamic response range of 0.005-50 ng mL-1 for cTnI targets with a detection limit of 1.3 pg mL-1, far superior to some reported test protocols. This work provides a designable pathway for the design of artificial enzymes with high enzyme-like activity to further expand the practical range of enzyme alternatives.
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