Antibacterial Activity and Mechanism of Action of Osthole against Listeria monocytogenes

单核细胞增生李斯特菌 细胞内 抗菌活性 细胞外 膜透性 化学 作用机理 ATP酶 细胞膜 细菌细胞结构 微生物学 细菌 细胞 细胞生物学 生物化学 生物 体外 遗传学
作者
Yang Kong,Hui Yan,Jinjing Hu,Yixuan Dang,Zihao Han,Bin Tian,Puxiu Wang
出处
期刊:Journal of Agricultural and Food Chemistry [American Chemical Society]
卷期号:72 (19): 10853-10861 被引量:10
标识
DOI:10.1021/acs.jafc.3c07931
摘要

The purpose of this study was to investigate the antibacterial activity and mechanism of action of osthole against Listeria monocytogenes. The antibacterial activity of osthole was evaluated by determining the minimum inhibitory concentration (MIC) and growth curve. Cell morphology, membrane permeability, membrane integrity, bacterial physiology, and metabolism were explored using different methods to elucidate the mechanism of action of osthole. It was shown that the MIC of osthole against L. monocytogenes was 62.5 μg/mL and it inhibited the growth of L. monocytogenes effectively in a concentration-dependent manner. Scanning electron microscopy (SEM) images demonstrated morphology changes of L. monocytogenes, including rough surface, cell shrinkage, and rupture. It was found that extracellular conductivity and macromolecule content were increased significantly in the presence of osthole, indicating the disruption of cell membrane integrity and permeability. Laser confocal microscopy results supported the conclusion that osthole caused severe damage to the cell membrane. It was also noticed that osthole depleted intracellular adenosine triphosphate (ATP), inhibited Na+-K+-ATPase and Ca2+-Mg2+-ATPase activity, and promoted the accumulation of intracellular reactive oxygen species (ROS), leading to cell death. This study suggests that osthole is a promising antibacterial agent candidate against L. monocytogenes, and it shows potential in the prevention and control of foodborne pathogens.
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