The SlCBL10 Calcineurin B-Like Protein Ensures Plant Growth under Salt Stress by Regulating Na+ and Ca2+ Homeostasis

拟南芥 液泡 突变体 木质部 拟南芥 分区(防火) 生物化学 细胞生物学 钠氢反转运蛋白 互补 共转运蛋白 焦磷酸酶 生物 龙葵 化学 基因 植物 运输机 细胞质 有机化学
作者
Isabel Egea,Benito Pineda,Ana Ortíz‐Atienza,Félix Plasencia,Stéphanie Drevensek,Begoña García‐Sogo,Fernando J. Yuste‐Lisbona,Javier Barrero‐Gil,Alejandro Atarés,Francisco Pardo,Frédy Barneche,Trinidad Angosto,Carmen Capel,Julio Salinas,Wim H. Vriezen,Elisabeth Esch,Chris Bowler,María C. Bolarín,Vicente Moreno,Rafael Lozano
出处
期刊:Plant Physiology [Oxford University Press]
卷期号:176 (2): 1676-1693 被引量:52
标识
DOI:10.1104/pp.17.01605
摘要

Characterization of a new tomato (Solanum lycopersicum) T-DNA mutant allowed for the isolation of the CALCINEURIN B-LIKE PROTEIN 10 (SlCBL10) gene whose lack of function was responsible for the severe alterations observed in the shoot apex and reproductive organs under salinity conditions. Physiological studies proved that SlCBL10 gene is required to maintain a proper low Na+/Ca2+ ratio in growing tissues allowing tomato growth under salt stress. Expression analysis of the main responsible genes for Na+ compartmentalization (i.e. Na+/H+ EXCHANGERs, SALT OVERLY SENSITIVE, HIGH-AFFINITY K+ TRANSPORTER 1;2, H+-pyrophosphatase AVP1 [SlAVP1] and V-ATPase [SlVHA-A1]) supported a reduced capacity to accumulate Na+ in Slcbl10 mutant leaves, which resulted in a lower uploading of Na+ from xylem, allowing the toxic ion to reach apex and flowers. Likewise, the tomato CATION EXCHANGER 1 and TWO-PORE CHANNEL 1 (SlTPC1), key genes for Ca2+ fluxes to the vacuole, showed abnormal expression in Slcbl10 plants indicating an impaired Ca2+ release from vacuole. Additionally, complementation assay revealed that SlCBL10 is a true ortholog of the Arabidopsis (Arabidopsis thaliana) CBL10 gene, supporting that the essential function of CBL10 is conserved in Arabidopsis and tomato. Together, the findings obtained in this study provide new insights into the function of SlCBL10 in salt stress tolerance. Thus, it is proposed that SlCBL10 mediates salt tolerance by regulating Na+ and Ca2+ fluxes in the vacuole, cooperating with the vacuolar cation channel SlTPC1 and the two vacuolar H+-pumps, SlAVP1 and SlVHA-A1, which in turn are revealed as potential targets of SlCBL10.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
yudada完成签到 ,获得积分10
刚刚
Hello应助缺叶酸的小狮子采纳,获得10
刚刚
KKK613完成签到,获得积分10
1秒前
笨笨歌曲完成签到,获得积分10
1秒前
吃菜菜发布了新的文献求助10
1秒前
科研通AI2S应助zhi采纳,获得10
1秒前
Dog完成签到,获得积分10
1秒前
哈哈哈发布了新的文献求助10
2秒前
蜡笔小心眼子完成签到,获得积分10
2秒前
潇洒的惋清应助gory采纳,获得10
2秒前
tangpc发布了新的文献求助10
2秒前
科研通AI6.4应助樱桃汽水采纳,获得10
2秒前
TINASO完成签到 ,获得积分10
3秒前
愉快的真发布了新的文献求助30
3秒前
野性的沛儿完成签到,获得积分10
4秒前
小医发布了新的文献求助20
4秒前
4秒前
4秒前
molihuakai应助liujiaying采纳,获得10
4秒前
2025完成签到 ,获得积分10
5秒前
万能图书馆应助丁丁采纳,获得10
5秒前
Nole应助byyyyy采纳,获得30
5秒前
6秒前
7秒前
认真幼萱应助苏桑焉采纳,获得10
9秒前
姜鸽完成签到,获得积分10
9秒前
科研通AI6.2应助崔崔采纳,获得10
9秒前
辛勤的梦曼完成签到,获得积分10
9秒前
行不行完成签到,获得积分10
9秒前
科研通AI6.4应助panpan采纳,获得10
10秒前
10秒前
wx完成签到,获得积分10
11秒前
11秒前
c嘉发布了新的文献求助10
11秒前
11秒前
12秒前
炙热从蕾发布了新的文献求助10
12秒前
spartanzhao发布了新的文献求助10
13秒前
13秒前
13秒前
高分求助中
Principles of Economics, 11th Edition 10000
University Physics with Modern Physics, 16th edition 10000
(应助此贴封号)【重要!!请各用户(尤其是新用户)详细阅读】【科研通的精品贴汇总】 10000
Development of a Bridge Weigh-In-Motion System: A technology to convert the bridge response to the passage of traffic into data on vehicle configurations, speeds, times of travel and weights 1000
Organic Reactions, Volume 116 1000
Current concepts in cutaneous toxicity : proceedings of the Fourth Conference on Cutaneous Toxicity, Washington, D.C., May 9-11, 1979 1000
ズームレンズの光学設計に関する研究 800
热门求助领域 (近24小时)
化学 材料科学 医学 生物 纳米技术 工程类 有机化学 化学工程 生物化学 计算机科学 内科学 物理 复合材料 催化作用 细胞生物学 无机化学 光电子学 物理化学 电极 基因
热门帖子
关注 科研通微信公众号,转发送积分 7278559
求助须知:如何正确求助?哪些是违规求助? 8899604
关于积分的说明 18822209
捐赠科研通 6950775
什么是DOI,文献DOI怎么找? 3206896
关于科研通互助平台的介绍 2377488
邀请新用户注册赠送积分活动 2181860