Expression of Cytokine-related genes and Oxido-reduction related genes using DNA microarray in Severe Preeclamptic Placenta. – microarray analysis of severe preeclamptic placenta

Objective: To investigate patterns of Cytokine-related genes and Oxido-reduction related gene expression in placental samples from patients with preeclampsia. Methods; Placenta from 13 normal pregnancies and 13 patients with preeclampsia were collected. Cytokine-related genes and oxido-reduction related genes were found using genome-wide expression profiling by DNA microarrays, followed by validation them using real-time PCR. Results: Among the 30,940 genes that were screened in the microarray, 515 genes were found to be differentially expressed between normal and preeclamptic placenta. Among these candidate genes, 121 were up-regulated and 121 were down-regulated. Gene ontology analysis revealed several biological processes associated with the development of preeclampsia, including angiogenesis, apoptosis, cell adhesion, cell cycle, cell differentiation, hypoxia, inflamatory response, metabolic process, signal transduction, transcription, transport, proteolysis. Among up-regulated genes, cytokine relates genes (Oncostatin M, VEGFA, FLT1) and Oxido-reduction related genes (LDHA, cyp26A1, SMOX) were selected for validation of microarray data. The microarray data also confirmed by the measurement of real-time PCR. Conclusion: DNA microarray is a great method to find candidate genes of preeclampsia and the candidate genes may prove to be novel biological markers and guidepost for elucidation of the pathogenesis. DNA microarray - Placenta - Preeclampsia