间充质干细胞
胰蛋白酶化
移植
细胞外基质
肌肉注射
细胞
材料科学
细胞外
细胞内
细胞生物学
体外
生物物理学
生物医学工程
化学
酶
医学
胰蛋白酶
生物
生物化学
外科
内科学
作者
Chun‐Hung Chen,Yen Chang,Chung-Chi Wang,Chih‐Hao Huang,Chieh‐Cheng Huang,Yi‐Chun Yeh,Shiaw-Min Hwang,Hsing‐Wen Sung
出处
期刊:Biomaterials
[Elsevier]
日期:2007-11-01
卷期号:28 (31): 4643-4651
被引量:49
标识
DOI:10.1016/j.biomaterials.2007.07.015
摘要
Cell transplantation via local intramuscular injection is a promising therapy. However, the cells used are usually expanded in vitro and dissociated by trypsinization, which may be harmful to the cells. In the study, a novel method for the construction of fragmented sheets of mesenchymal stem cells (MSCs) with a uniform size, without treating with any enzymes, was reported. The obtained MSC sheets preserved the intercellular junctions and endogenous extracellular matrix and kept their cell phenotype. After injection through a needle, the fragmented MSC sheets maintained intact and retained their activity upon transferring to another growth surface, while the complete cell sheets were torn into pieces. Transplantation of fragmented MSC sheets in the skeletal muscle of a syngeneic rat model via local injection was evaluated. The transplanted MSC sheets were mainly localized at the site of injection, while the dissociated MSCs were scattered around. Additionally, there were significantly more MSCs retained in the local skeletal muscle for the group injected with fragmented MSC sheets than that injected with dissociated MSCs. These results indicated that the fragmented cell sheets might be used as a novel therapeutic cell-carrier for intramuscular administration.
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