转基因
生物
转基因小鼠
功能(生物学)
异位表达
细胞生物学
野生型
细胞培养
基因
遗传学
突变体
作者
Lisa Nunez,Sharada Mokkapati,Cheng-Tai Yu,Jian Min Deng,Richard R. Behringer,Angabin Matin
出处
期刊:Genesis
[Wiley]
日期:2019-09-12
卷期号:57 (11-12)
被引量:2
摘要
Summary Dead‐End 1 (DND1) encodes an RNA binding protein critical for viable primordial germ cells in vertebrates. When introduced into cancer cell lines, DND1 suppresses cell proliferation and enhances apoptosis. However, the molecular function of mammalian wild‐type DND1 has mostly been studied in cell lines and not verified in the organism. To facilitate study of wild‐type DND1 function in mammalian systems, we generated a novel transgenic mouse line, LSL‐FM‐DND1 flox/+ , which conditionally expresses genetically engineered, FLAG‐tagged and myc‐tagged DND1 in a cell type‐specific manner. We report that FLAG‐myc‐DND1 is indeed expressed in specific tissues of the mouse when LSL‐FM‐DND1 flox/+ is combined with mouse strains expressing Cre‐recombinase . LSL‐FM‐DND1 flox/+ mice are fertile with no overt health effects. We expressed FLAG‐myc‐DND1 in the pancreas and found that chronic, ectopic expression of FLAG‐myc‐DND1 led to increase in fasting glucose levels in older mice. Thus, this novel LSL‐FM‐DND1 flox/+ mouse strain will facilitate studies on the biological and molecular function of wild‐type DND1.
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