The method to detect mesotrione residue and the residual dynamics of mesotrione in maize plants,maize seeds and the soil was studied.The results showed that the minimum detectable amount of mesotrione was 0.3 ng.The minimum detectable concentrations of mesotrione in maize plants,maize seeds and the soil were 0.02 mg/kg,0.01 mg/kg and 0.01 mg/kg,respectively.The fortified recoveries of mesotrione in maize plants,maize seeds and the soil were 80.94%-89.0%,78.11%-87.15% and 81.27%-95.38%,with the relatively standard deviation 3.66%-12.54%,3.95%-11.54% and 4.25%-6.28%,respectively.The half-life periods of mesotrione in maize plants and the soil were 5.6-6.8 days and 10.4-10.8 days,respectively.