SCREENING OF EFFECTUAL SIRNA-MEDIATED TRF2 BY REAL-TIME FLUORESCENCE QUANTITATIVE PCR

[Objective] To screen effectual siRNA-mediated TRF2,and lay the foundation for further studying on antiTRF2 by RNA interfere. [Methods] Three TRF2-specific double stranded siRNAs were designed targeting different regions of TRF2 mRNA by chemic method,siRNAs were electroporated into MCF-7 cells by LipofectamineTM2000,TRF2 mRNA expression level were determined by Real-time fluorescence quantitative RT-PCR. [Results] Two of the three customized TRF2-siRNAs could effectively inhibit the expression of TRF2 mRNA. [Conclusion] siRNA synthesizing with chemic method and determining gene expression with Real-time fluorescence quantitative RT-PCR could rapidly,specifically and effectively screen siRNA which could suppress the expression of TRF2 mRNA.