Objective To study the therapeutic effect of vaginoplasty using sigmoid colon graft for congenital absence of vagina. Method 12 cases of congenital absence of vagina were treated with vaginoplasty using sigmoid colon from May 2002 to August 2005. Results All cases were succeeded. The vagina could contain 2 fingers with a depth of 10 to 13 cm. 3 cases developed stricture 2 months postoperatively and improved by dilation. Within 6 months to 3 years follow-up, 11 cases had sex intercourse of which 10 cases were satisfied and 1 case was dissatisfied. Conlusion Vaginoplasty using sigmoid colon graft for congenital absence of vagina is effective, and dilation within 2 months postoperatively is recommendable to prevent stricture.