Improvement of MTT assay by 2-chloroadenosine in activation test and cytotoxicity test of lymphocyte

Objective:To sutdy the effect of 2 chloroadenosine(2 ClA),which is specifically cytotoxic to macrophages,on MTT assay in activation test and cytotoxicity test of lymphocyte.Methods:Using cell culture technique,mouse splenic lymphocytes and peritoneal macrophages were cultured.Lymphocyte activation and specific cytotoxicity to tumor cells and toxicity of 2 ClA to macrophages were measured by MTT assay in the presence or absence of 2 ClA.Results:2 ClA had a strong cytotoxic effect on macrophages.When the activation test and cytotoxicity test of lymphocyte were measured by MTT assay,the optical density values of 2 ClA group was lower than that of control group,and statistic analysis showed P0 05.Conclusion:With the specific cytotoxicity to macrophages,2 ClA can exclude the influence of macrophages to MTT assay in activation test and cytotoxicity test of lymphocyte,and more reliable results will be obtained.