Optimization of the Ethanol Extraction Technology of Hyperin from Hypericum perforatum by Orthogonal Experiment

OBJECTIVE:To optimize the ethanol extraction technology of hyperoside from Hypericum perforatum by orthogonal experiment.METHODS:The extraction technology of hyperoside from H.perforatum was optimized by orthogonal experiment using ethanol concentration,ethanol ratio,extraction temperature,extraction time as factors and the ertraction rate of hyperoside as index.RESULTS:The optimal extraction technology was as follows:20-folds of 60% ethanol,at temperature of 90℃ for 4 times,2 hours each time.CONCLUSION:The method is simple,accurate,reproducible,and can be used as the extraction technology of hyperoside from H.perforatum.