An attempt to establish a methamidophos-induced delayed neurotoxicity model in Beijing White Laying Hens was performed. Neuropathy target esterase (NTE) inhibition was evaluated. It was showed that surviving hens exposed to methamidophos did not develop organophosphate-induced delayed neurotoxicity. The results indicate that delayed neurotoxicity is not induced by methamidophos administered orally and subcutaneously in adult hens.