去细胞化
细胞外基质
再生(生物学)
周围神经
细胞外
化学
组织工程
免疫组织化学
生物医学工程
解剖
细胞生物学
病理
医学
生物
生物化学
作者
Terry W. Hudson,Scott A. Zawko,Curt Deister,Scott Lundy,Chaofang Hu,Kate Lee,Christine E. Schmidt
出处
期刊:Tissue Engineering
[Mary Ann Liebert]
日期:2004-11-01
卷期号:10 (11-12): 1641-1651
被引量:304
标识
DOI:10.1089/ten.2004.10.1641
摘要
The long-term goal of our research is to engineer an acellular nerve graft for clinical nerve repair and for use as a model system with which to study nerve-extracellular matrix interactions during nerve regeneration. To develop this model acellular nerve graft we (1) examined the effects of detergents on peripheral nerve tissue, and (2) used that knowledge to create a nerve graft devoid of cells with a well-preserved extracellular matrix. Using histochemistry and Western analysis, the impact of each detergent on cellular and extracellular tissue components was determined. An optimized protocol was created with the detergents Triton X-200, sulfobetaine-16, and sulfobetaine-10. This study represents the most comprehensive examination to date of the effects of detergents on peripheral nerve tissue morphology and protein composition. Also presented is an improved chemical decellularization protocol that preserves the internal structure of native nerve more than the predominant current protocol.
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