Biodegradation of the Fungicide Picoxystrobin by Hyphomicrobium sp. H-9 and Detoxification Mechanism as Indicated by the Microalgae Tetradesmus obliquus

Picoxystrobin is widely used in agriculture, causing residual contamination of habitats and ecotoxicity. In this study, a picoxystrobin-degrading strain H-9 was isolated and identified as Hyphomicrobium sp. It can rapidly degrade four strobilurin fungicides (picoxystrobin, trifloxystrobin, azoxystrobin, and kresoxim-methyl) to produce the corresponding SF acids and methanol via the hydrolysis of ester bonds. Picoxystrobin can significantly inhibit the growth of algal cells and the synthesis of chlorophyll in Tetradesmus obliquus. The detoxification capacity of strain H-9 was evident as it mitigated picoxystrobin-induced growth inhibition and oxidative stress in T. obliquus. Strain H-9 modulated the expression of genes involved in photosynthesis, the Calvin cycle, the tricarboxylic acid cycle, glycolysis, oxidative stress, and chlorophyll synthesis, thereby alleviating picoxystrobin toxicity to T. obliquus. This study enhances our understanding of the detoxification mechanism of microorganisms on microalgae exposed to SF stress and provides a novel insight into the microbial remediation of SFs-contaminated environments.