Folate Biosynthesis is Boosted in Legume Nodules

Symbiotic nitrogen fixation (SNF) profoundly alters plant and bacteroid metabolism; however, SNF impact on folates and one-carbon (1C) metabolism are unknown. To explore this, SNF was induced in Phaseolus Vulgaris with Rhizobium etli. Nodules accumulated the highest folate concentration yet reported in a plant tissue (60 nmol/g fresh weight). Folate upregulation was not exclusive of determinate nodules, moderate to high folate contents were also encounter in Medicago truncatula and sativa. Moreover, folates correlated partial and positively with N