Systematic Profile of Oxylipins in Myocardial Infarction by Liquid Chromatography–Tandem Mass Spectrometry

ABSTRACT Rationale Oxylipins play an important role in inflammatory processes, accompanying the occurrence of myocardial infarction. Analyzing a wide panel of oxylipins derived from more polyunsaturated fatty acids may provide valuable information to elucidate the relationships between the signaling mediator profile and myocardial infarction comprehensively. Methods An ultrahigh‐performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) method for simultaneously measuring 74 oxylipins in 50‐μL human samples with a 10‐min chromatographic run and easy operation has been developed and validated. Results Accuracy and precision were below 15% of the relative errors in 99% of quality control. Recoveries and matrix effect were considered acceptable. Potential effects of different collecting tubes were also assessed. We successfully utilized our approach to measure plasma samples obtained from 99 healthy individuals and 302 myocardial infarction patients. Conclusions Profiles of oxylipins discovered potential biomarkers and clarified the pathological characteristics of oxylipin metabolism in myocardial infarction. Our approach was rapid, accurate, and precise, with high throughput, low sample volume, and easy operation, suitable for large‐scale studies.