邻苯二甲酸盐
生殖细胞
生物
减数分裂
细胞生物学
转录组
DNA损伤
遗传学
基因
男科
DNA
基因表达
化学
医学
有机化学
作者
Yu Tian,Ye Zhang,Pei-Yu Dong,Yongjun Sun,Ai-Hong Zhao,Wei Shen,Xifeng Zhang
出处
期刊:Chemosphere
[Elsevier]
日期:2022-11-01
卷期号:307: 135698-135698
被引量:6
标识
DOI:10.1016/j.chemosphere.2022.135698
摘要
Di(2-ethylhexyl)phthalate (DEHP) has proven characteristics of an endocrine-disrupting compound (EDC), which can threaten the reproductive health of humans and other animals. In mammals, a series of chromosomal events occur during the meiotic stage of oocytes. External toxins may enter the body and cause infertility and other related diseases. Therefore, it is crucial to explore the influence of DEHP exposure on the molecular mechanism of germ cell meiosis. We used single-cell RNA sequencing (scRNA-seq) to analyse the ovaries of foetal mice at embryonic day 12.5 (E12.5) and E14.5 after maternal DEHP exposure. DEHP exposure further activated the pathways related to DNA repair in germ cells, increased the expression of genes related to DNA damage and changed the developmental trajectory of germ cells. DEHP exposure may affect the proliferation of pregranulosa (PG) cells. Moreover, DEHP exposure altered the signal transduction between PG cells and germ cells. We showed that DEHP affects meiosis by causing DNA damage in oocytes and disrupting the signal transduction between PG cells and germ cells. These results provide a strong theoretical basis for the prevention and treatment of DEHP-mediated female reproductive health problems.
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