上睑下垂
生物
T细胞
程序性细胞死亡
流式细胞术
质量细胞仪
表型
细胞
免疫学
效应器
细胞生物学
病毒学
基因
细胞凋亡
遗传学
免疫系统
作者
Xiaoyu Luo,Julie Frouard,Gang Zhang,Jason Neidleman,Guorui Xie,Emma Sheedy,Nadia R. Roan,Warner C. Greene
标识
DOI:10.3389/fimmu.2022.883420
摘要
CD4 T lymphocytes belong to diverse cellular subsets whose sensitivity or resistance to HIV-associated killing remains to be defined. Working with lymphoid cells from human tonsils, we characterized the HIV-associated depletion of various CD4 T cell subsets using mass cytometry and single-cell RNA-seq. CD4 T cell subsets preferentially killed by HIV are phenotypically distinct from those resistant to HIV-associated cell death, in a manner not fully accounted for by their susceptibility to productive infection. Preferentially-killed subsets express CXCR5 and CXCR4 while preferentially-infected subsets exhibit an activated and exhausted effector memory cell phenotype. Single-cell RNA-seq analysis reveals that the subsets of preferentially-killed cells express genes favoring abortive infection and pyroptosis. These studies emphasize a complex interplay between HIV and distinct tissue-based CD4 T cell subsets, and the important contribution of abortive infection and inflammatory programmed cell death to the overall depletion of CD4 T cells that accompanies untreated HIV infection.
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