PDZ域
人类蛋白质组计划
计算机科学
领域(数学分析)
人类蛋白质
计算生物学
生物系统
生物
纳米技术
化学
材料科学
细胞生物学
数学
生物化学
蛋白质组学
基因
数学分析
作者
A. Cousido-Siah,Laura Carneiro,Camille Kostmann,Péter Ecsédi,László Nyitray,Gilles Travé,Gergő Gógl
出处
期刊:Acta Crystallographica Section D: Structural Biology
[Wiley]
日期:2022-03-11
卷期号:78 (4): 509-516
被引量:3
标识
DOI:10.1107/s2059798322001784
摘要
The human PDZome represents one of the largest globular domain families in the human proteome, with 266 instances. These globular domains typically interact with C-terminal peptide motifs found in thousands of human proteins. Despite previous efforts, not all PDZ domains have experimentally solved structures and most of their complexes remain to be solved. Here, a simple and cost-effective strategy is proposed for the crystallization of PDZ domains and their complexes. A human annexin A2 fusion tag was used as a crystallization chaperone and the structures of nine PDZ domains were solved, including five domains that had not yet been solved. Finally, these novel experimental structures were compared with AlphaFold predictions and it is speculated how predictions and experimental methods could cooperate in order to investigate the structural landscapes of entire domain families and interactomes.
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