| 标题 |
Novel mouse uterine natural killer cell subsets expressing cd8a receptor related to cytotoxic response
表达与细胞毒性反应相关的cd8a受体的新型小鼠子宫自然杀伤细胞亚群
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| 其它 | Materials and methods: Uterine mesometrial region from pregnant mice on gestational day (gd) 9th were dissected ad uNK cells were isolated using Dolichosbiflorus agglutinin (DBA) lectin coated magnetic beads of gradient of Percoll centrifugation. The isolated cells were labeled with fluorochrome conjugated anti-NK1. 1, anti-CD8a, anti-CD14, anti-CD3 and DBA lectin for single, double and triple labeling to be quantified using fluorescence activated cell sorting (FACS). In parallel uterine fragments were processed for histological sections and immunostained with anti-perforin and DBA lectin cytochemistry for morphometry |
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