代谢组
转录组
丁酸钠
微生物群
生物
丁酸盐
代谢组学
果蝇属(亚属)
肠道菌群
寄主(生物学)
基因组
计算生物学
肠道微生物群
代谢途径
微生物代谢
蛋白质组学
遗传学
基因
生物化学
生物信息学
基因表达
发酵
作者
Fan Zhou,Biao-Di Liu,Xin Liu,Yan Li,Luoluo Wang,Jian Huang,Guan‐Zheng Luo,Xiaoyun Wang
出处
期刊:Metabolites
[MDPI AG]
日期:2021-05-06
卷期号:11 (5): 298-298
被引量:10
标识
DOI:10.3390/metabo11050298
摘要
The host microbiome plays an important role in regulating physiology through microbiota-derived metabolites during host-microbiome interactions. However, molecular mechanism underly host-microbiome interactions remains to be explored. In this study, we used Drosophila as the model to investigate the influence of microbiome and microbiota-derived metabolite sodium butyrate on host transcriptome and metabolome. We established both a sterile Drosophila model and a conventional Drosophila model to demonstrate the role of sodium butyrate. Using multi-omics analysis, we found that microbiome and sodium butyrate could impact host gene expression patterns in both the sterile Drosophila model and the conventional Drosophila model. The analysis of gut microbial using 16S rRNA sequencing showed sodium butyrate treatment also influenced Drosophila bacterial structures. In addition, Drosophila metabolites identified by ultra-high performance liquid chromatography-MS/MS were shown to be affected by sodium butyrate treatment with lipids as the dominant changed components. Our integrative analysis of the transcriptome, the microbiome, and the metabolome data identified candidate transcripts that are coregulated by sodium butyrate. Taken together, our results reveal the impact of the microbiome and microbiota-derived sodium butyrate on host transcriptome and metabolome, and our work provides a better understanding of host-microbiome interactions at the molecular level with multi-omics data.
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