Wide temperature adaptive oxidase-like based on mesoporous manganese based metal-organic framework for detecting total antioxidant capacity

Overcoming the intense variation of enzymatic activity among different temperatures is very critical in catalytic medicine and catalytic biology. Here, Mn-based metal-organic framework-based wide-temperature-adaptive mesoporous artificial enzymes (Mn-TMA-MOF) were designed and synthesized. The oxidase-like Mn-TMA-MOF showed excellent catalytic activity at 0-50 °C and avoided the activity loss and instability due to temperature variation that occurred. The excellent oxidase-like properties of Mn-TMA-MOF with wide temperature adaptativeness are mainly ascribed to the mixed oxidized state (Mn3+/Mn2+) and high substrate affinity (Km = 0.034 mM) of Mn. Moreover, the mesopore-micropores two-level structure of Mn-TMA-MOF provides a large space and surface area for enzyme catalysis. Based on the stability of Mn-TMA-MOF, we developed a colorimetric sensor that can detect total antioxidant capacity in fruits with a limit of detection up to 0.59 μM.