病毒潜伏期
Jurkat细胞
延迟(音频)
病毒学
前病毒
生物
病毒复制
人类免疫缺陷病毒(HIV)
离体
细胞生物学
抗逆转录病毒疗法
瓶颈
体内
病毒
免疫学
绿色荧光蛋白
活体细胞成像
细胞培养
病毒载量
T细胞
遗传学
基因组
计算机科学
基因
免疫系统
嵌入式系统
电信
作者
Cai Jinfeng,Hongbo Gao,Jiacong Zhao,Shujing Hu,Xinyu Liang,Yanyan Yang,Zhuanglin Dai,Zhongsi Hong,Kai Deng
出处
期刊:eLife
[eLife Sciences Publications, Ltd.]
日期:2021-04-09
卷期号:10
被引量:6
摘要
The major barrier to curing HIV-1 infection is a small pool of latently infected cells that harbor replication-competent viruses, which are widely considered the origin of viral rebound when antiretroviral therapy (ART) is interrupted. The difficulty in distinguishing latently infected cells from the vast majority of uninfected cells has represented a significant bottleneck precluding comprehensive understandings of HIV-1 latency. Here we reported and validated a newly designed dual fluorescent reporter virus, DFV-B, infection with which primary CD4 + T cells can directly label latently infected cells and generate a latency model that was highly physiological relevant. Applying DFV-B infection in Jurkat T cells, we generated a stable cell line model of HIV-1 latency with diverse viral integration sites. High-throughput compound screening with this model identified ACY-1215 as a potent latency reversing agent, which could be verified in other cell models and in primary CD4 + T cells from ART-suppressed individuals ex vivo. In summary, we have generated a meaningful and feasible model to directly study latently infected cells, which could open up new avenues to explore the critical events of HIV-1 latency and become a valuable tool for the research of AIDS functional cure.
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