胡桃醌
细胞毒性
细胞毒性T细胞
细胞生长
细胞培养
活力测定
程序性细胞死亡
过氧化氢酶
细胞内
体外
胶质瘤
化学
生物
癌症研究
药理学
细胞凋亡
生物化学
氧化应激
遗传学
作者
Džiugas Meškelevičius,Kastytis Šidlauskas,Rūta Bagdonavičiūtė,Julius Liobikas,Daiva Majienė
出处
期刊:Anti-cancer Agents in Medicinal Chemistry
[Bentham Science]
日期:2016-08-16
卷期号:16 (9): 1190-1197
被引量:14
标识
DOI:10.2174/1871520616666160204113217
摘要
In this study we evaluated the impact of juglone on rat glioma C6 cell culture viability, proliferation and invasiveness in vitro. Juglone induced C6 cell death with EC50 concentrations equal to 10.4 ± 1.6 µM after 24h incubation. At relatively low concentrations juglone significantly decreased cell proliferation, reduced spheroid invasiveness and suppressed "wound" healing. In addition, generation of intracellular reactive oxygen and nitrogen species (RS) was detected in cells treated with juglone. Noteworthy, juglone was relatively stable in cell culture medium and levels of H2O2 generated from juglone due to its probable reaction with medium components were not sufficient to affect the viability of glioma cells. Moreover, addition of catalase to the cell medium did not reduce the cytotoxicity of juglone. Therefore, we propose that cell death may be induced through the action of RS other than H2O2, However the direct effect of juglone on the cellular targets could not be excluded either. In conclusion, juglone exerted cytotoxic, anti-proliferative and anti-invasive effects on C6 rat glioma cells in vitro.
科研通智能强力驱动
Strongly Powered by AbleSci AI