The feasibility study of diagnostic ultrasound in presence of microbubbles for disruption of blood bram-barrier:microbubbles destruction experiment in vitro

Objective To explore the feasibility of microbubbles destruction with diagnostic ultrasound irradiation through the human cranial bone,and to investigate the condition for further disruption of the blood brain barrier in vivo with diagnostic ultrasound and microbubbles.Methods Microbubbles destruction experimental facility was established in vitro.The GE Vivid 7,Sequoia 512 and Philips iE 33 ultrasound equipments were applied for destruction of microbubbles through different parts of skulls(os temporale,occipital bone,bregmatic bone)with different ages at the depth of 5.0 cm.Sequoia 512 irradiation of themicrobubbles with the lowest frequency(1.75 MHz) and maximum mechanical index through the young os temporale was performed to analyze the microbubbles destruction in different irradiation depth.Results The microbubbles were destructed conspicuously by three types of diasonographies exposure through the puerile,young and senile os temporale and occipital bone except through bregmatic bone.At the depth of 7.5 cm,Sequoia 512 diasonography irradiation could still significantly destruct the microbubbles.Conclusion Diagnostic ultrasound associated with microbubbles might open the blood-brain barrier.