S-nitrosoglutathione inhibits cardiaomyocyte hypertrophy induced by prostaglandin F2α

Objective To observe the effects of S-nitrosoglutathione(GSNO)on cardiac myocyte hypertrophy induced by prostaglandin F2α(PGF2α),and to probe primarily its mechanisms.Methods Neonatal rat cardiomyocyte hypertrophic response and the antihypertrophic effects of GSNO were assayed by measuring cell diameter and protein content.The nitric oxide(NO) concentration and the intracellular free calcium concentration((Ca2+)i) in cultured cardiomyocytes were measured by colorimetry or by using Fura 2/AM as a fluorescent indicator,respectively.Results PGF2α at 10-7mol/L significantly increased the cell diameter,protein content and(Ca2+)i in cultured cardiomyocyte,but had no effects on NO release.GSNO at 10-4mol/L could significantly inhibit the cardiomyocyte hypertrophy induced by PGF2α.Oppositely,NO release was reduced by GSNO(P0.05).Similarly,(Ca2+)i was reduced by GSNO.Conclusion Cardiomyocyte hypertrophyic response could be abolished by GSNO partly,which may be related to its effects on the increase the release of NO and then inhibiting the increase of(Ca2+)i induced by PGF2α.