化学
适体
检出限
表面增强拉曼光谱
拉曼光谱
胶体金
色谱法
卡那霉素
分析化学(期刊)
纳米颗粒
残留物(化学)
纳米技术
拉曼散射
生物化学
分子生物学
物理
材料科学
光学
生物
基因
作者
Yuan Jiang,Da‐Wen Sun,Hongbin Pu,Qingyi Wei
出处
期刊:Talanta
[Elsevier]
日期:2019-05-01
卷期号:197: 151-158
被引量:120
标识
DOI:10.1016/j.talanta.2019.01.015
摘要
An ultrasensitive method for the kanamycin (KANA) detection in milk sample using surface-enhanced Raman spectroscopy-based aptasensor was employed in the current study. Double strand DNA binding bimetallic gold@silver nanoparticles were developed as a sensing platform. Probe DNAs were first embedded on the surface of gold nanoparticles by the end-modified thiol, and after silver shell encapsulating, KANA aptamer DNAs with the Raman reporter Cy3 were then hybridized with probe DNAs by complementary base pairing. Results showed that with increase in the KANA concentration, the Raman intensity of Cy3 decreased. Besides achieving selectivity, an ultralow detection limit of 0.90 pg/mL, a broad linear relationship ranging from 10 μg/mL to 100 ng/mL in aqueous reagent and satisfactory recoveries of 90.4-112% in liquid whole milk were obtained. The result of actual sample proved that this aptasensor was promising in trace determination of KANA residue.
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